![]() In third aspect, there is provided a method of growing and/or culturing a cell, wherein the method comprises growing and/or culturing a cell in the serum-free cell culture medium of the first aspect or the second aspect. In second aspect, there is provided a serum-free cell culture medium comprising maltose and at least one, at least two, at least three or more saccharides as carbohydrate sources. The cell culture medium comprises maltose as sole carbohydrate source. In first aspect, there is provided a serum-free cell culture medium. In particular, the potential use of alternative carbohydrate source may lead to the improvement of carbohydrate loading in the batch culture medium, and possibly lead to the decrease lactate accumulation which may become toxic to cells.Īccordingly, there is a need to provide a cell culture medium that has an alternative carbohydrate source. The potential use of alternative carbohydrate source, as energy source can have practical implications in biopharmaceutical manufacturing. ![]() However, one that remains a constant for cells (such as mammalian cells) are the provision of glucose as a carbohydrate source.Īs such, there is a need to provide cell culture medium that provides alternative carbohydrate source. The first few cell culture solution provides irrigation, supply of water, inorganic anions required for cell metabolism, osmotic balance, a buffering system, and a carbohydrate source for growth.Īs research into how cells function improves, cell culture medium has evolved to include multitude of components, which may be included depending on the purpose of the medium. BACKGROUND OF THE INVENTIONįrom the first day of its inception, cell culture medium has progressively been improved and researched depending on the intention of the culture. The present invention also relates to compositions for supporting protein production by cultured cells. In particular, the present invention relates to culture media. The present invention relates to compositions for propagation, preservation, or maintenance of cells. 2016, the contents of it being hereby incorporated by reference in its entirety for all purposes. ![]() This application claims the benefit of priority of Singapore patent application No.
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